Cat. #151447
CMT 64 Cell Line
Cat. #: 151447
Unit size: 1x10^6 cells / vial
Organism: Mouse
Tissue: Lung
Disease: Cancer
Model: Cancer cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Riddle
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Primary Citation: Franks et al. 1976. Cancer Res. 36(3):1049-1055. PMID: 1253168.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: CMT 64 Cell Line
- Alternate name: CMT-64; CMT.64; CMT 64; C57 Mouse Tumor 64
- Cancer type filter: Lung cancer
- Cancers detailed: Lung carcinoma;Metastatic
- Research fields: Cancer;Drug development
- Organism: Mouse
- Gender: Female
- Tissue: Lung
- Disease: Cancer
- Growth properties: Adherent
- Model: Cancer cell line
- Description: The tumourigenic and metastatic CMT 64 murine cell line is an in vivo mouse tumourigenesis system to study the growth characteristics and metastasis of mouse tumour lines (homoplastic with stable CMT 167 line); it demonstrates stable growth characteristics and morphology in culture and in lung metastasis induced after subcutaneous inoculation of mice. This demonstrates its consistency and reliability as a model for studying lung carcinoma progression and metastasis.
- Production details: CMT 64 was isolated from a primary alveogenic lung carcinoma tumour in a C57BL/1CRF mouse. The cell line maintained a stable morphology and growth rate comparable to that observed in the original tumour tissue in vitro. Notably, CMT 64 retained similar characteristics in both primary tumour growth and in lung metastases following subcutaneous inoculation in mice.
- Cellosaurus id: CVCL_2406
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10^5 cells/cm². Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Related Tools
- Related tools: CMT 64/61 Cell Line ; CMT 170 Cell Line
References
- Miyashita N et al. 2021. Sci Rep. 17:11(1):22380. PMID: 34789779
- Rincon et al. 2017. Oncotarget. : PMID: 28525366
- Franks et al. 1976. Cancer Res. 36(3):1049-1055. PMID: 1253168
