Cat. #160886
Recombinant CHO-K1 cell line with integrated Technology for enhanced production of second generation Human Erythropoietin protein called Darbepoetin (Darbe), in Adherent Cells
Cat. #: 160886
Availability: 10-12 weeks
Organism: Hamster
Tissue: Ovary
Disease: Therapeutics
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Saiyed Taslimarif ; Patel Paresh Brijalal ; Mukhopadhyaya Rabindranath
Institute: The Centre for Cellular and Molecular Platforms C-CAMP
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Recombinant CHO-K1 cell line with integrated Technology for enhanced production of second generation Human Erythropoietin protein called Darbepoetin (Darbe), in Adherent Cells
- Alternate name: haematopoietin, or haemopoietin
- Research fields: Drug development
- Parental cell: CHO-K1
- Organism: Hamster
- Tissue: Ovary
- Donor: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbe gene and 2) a ShRNA targeting a specific protease that can cleave the Darbe protein. G418 and Puromycin were used for selection of stable clones. The Darbe is expressed without any affinity tag and is actively secreted in the culture supernatant.
- Disease: Therapeutics
- Conditional: No
- Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbe gene and 2) a ShRNA targeting a specific protease that can cleave the Darbe protein. G418 and Puromycin were used for selection of stable clones. The Darbe is expressed without any affinity tag and is actively secreted in the culture supernatant.
Target Details
- Target: Erythropoietin (EPO), Darbepoetin (Darbe)
Handling
- Growth medium: F12 media supplemented with 10% FBS and 1% of 5000U/ml Penstrep (penicillin G and streptomycin) antibiotic. The cells are grown in a CO2 incubator at 37?°C with 5% CO2.
- Temperature: 37° C
- Atmosphere: 5% CO2
References
- A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)
