Cat. #160717
CHO DC-SIGN-N80A cell line
Cat. #: 160717
Unit size: 1x10^6 cells / vial
Organism: Hamster
Tissue: Ovary
Disease: HIV-1
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Alessandra Cambi
Institute: Radboud University Medical Center
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: CHO DC-SIGN-N80A cell line
- Alternate name: DC-SIGN N8A
- Research fields: Cell signaling and signal transduction;Immunology
- Parental cell: CHO
- Organism: Hamster
- Tissue: Ovary
- Disease: HIV-1
- Conditional: No
- Description: Dendritic Cell-Specific ICAM-3-Grabbing Non-Integrin (DC-SIGN; CD209) is a type II plasma membrane PRR abundantly expressed in antigen-presenting cells such as dendritic cells (DCs) and activated macrophages. As member of the C-type Lectin Receptor (CLR) family, DC-SIGN binds a plethora of pathogens, ranging from viruses like HIV-1, ebola virus, and hepatitis C virus, to larger pathogens like Mycobacterium tuberculosis and Candida albicans. This N80A mutant lacks the N-glycosylation ligand binding domain.
- Production details: CHO cell lines stably expressing DC-SIGN-N80 were established by Lipofectamin 2000 transfection, and were cultured in HamÄË?Â?Âs F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5ÄË?Â?Âmg/ml of the aminoglycoside antibiotic G418.
Target Details
- Target: Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin lacking the ligand binding domain N80.
Handling
- Format: Frozen
- Growth medium: HamÄË?Â?Âs F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5ÄË?Â?Âmg/ml of the aminoglycoside antibiotic G418.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: CHO DC-SIGN WT cell line ; CHO DC-SIGN-ÄÂ?ÂRep cell line ; CHO DC-SIGN-ÄÂ?ÂCRD cell line
References
- Te Riet et al. 2017. Sci Rep. 7(1):6713. PMID: 28751750
