Cat. #152843
BICR 18 Cell Line
Cat. #: 152843
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Larynx
Disease: Cancer
Model: Tumourigenic cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Eric Kenneth Parkinson
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BICR 18 Cell Line
- Alternate name: bicr18; BICR-18; BICR18; Beatson Institute for Cancer Research 18; HNSCC; SCC-HN
- Cancer: Head and neck cancer
- Cancers detailed: Larynx squamous cell carcinoma (lymph node metastasis)
- Research fields: Apoptosis and autophagy;Cancer;Drug development;Genetics
- Organism: Human
- Gender: Male
- Tissue: Larynx
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumourigenic cell line
- Conditional: Yes
- Description: Adherent keratinocyte cell line derived from a lymph node metastasis squamous cell carcinoma (SSC) of the larynx. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
- Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 8,13 D16S539: 12,14 D5S818: 12 D7S820: 9 THO1: 7 TPOX: 9,12 vWA: 17,20
- Cellosaurus id: CVCL_2309
Applications
- Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 8,13 D16S539: 12,14 D5S818: 12 D7S820: 9 THO1: 7 TPOX: 9,12 vWA: 17,20
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density of 4 x 10,000 cells/cm². Use of conditioned medium at a ratio of 1:1 with fresh medium can improve cell condition. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells. Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10^4 cells/cm².
Related Tools
- Related tools: BICR 10 Cell Line ; BICR 16 Cell Line ; BICR 3 Cell Line ; BICR 56 Cell Line ; BICR 6 Cell Line ; BICR 78 Cell Line ; BICR 22 Cell Line ; BICR 31 Cell Line ; BICR 82 Cell Line
References
- Loughran et al. 1997. Oncogene. 14(16):1955-1964. PMID: 9150362.
- Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.
