A2780cis Cell Line

The cisplatin-resistant A2780cis cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line

Contributors

Inventor Institute
Timothy Ward National Cancer Institute
Cat. #: 152708
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Ovarian cancer
Organism: Human
Tissue: Ovary
Gender: Female
Model: Tumour line
Morphology: Epithelial
Growth properties: Adherant
Cancer Types In Detail: Human ovarian carcinoma;Human ovarian carcinoma
Alternate name: A2780 cis; A2780/cis; A2780-cis; A2780CIS; A2780cis Cells; cisplatin-resistant cell line
Product description: A2780 cell line resistant to cisplatin. Background and Research Application The cisplatin-resistant A2780cis cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (catalogue no. 152706) to increasing concentrations of cisplatin. This cell line was derived from a patient with ovarian endometroid adenocarcinoma. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatin has to be added to the media for every passage. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (catalogue no. 152707), has been isolated from the same parental line. A2780cis has been used in a variety of experiments, including studying the cytotoxic effects of platinum (II) complexes and gold (I)-triphenylphosphine complexes with hypoxanthine-derived ligands. It has also been used to study the effects of cancer-cell specific oligopeptides.
Gender: Female
CRISPR: Yes
Conditional: Yes
Production details: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 1×1,000 to 1×10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.
Additional notes: Points of Interest This cell line has also been used in toxicity testing and drug resistance studies. Karyotype: Modal no. 46 STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,13 D5S818: 11 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16 Concentration Vial has between 1-5 million cells as standard, however this may vary.
Cellosaurus ID: CVCL_1942
Parental cell line: A2780
disease: Cancer;Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 + 2mM Glutamine + 1µM cisplatinum + 10% Foetal Bovine Serum (FBS). To retain resistance cisplatin must be added to the media every 2-3 passages.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x10,000cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.
Mycoplasma free: Yes
References:

Wang et al. 2015. Theranostics. 5(4): 431-442. PMID: 25699101.

Starha et al. 2014. PLoS One. 9(3):e90341. PMID: 24603594

Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.

Marcotte et al. 2012. Cancer Discov. 2(2):172-189. PMID: 22585861.

Rothenberg et al. 2010. Cancer Res. 70(6):2158-2164. PMID: 20215515.

Masuda et al. 1988. Cancer Res. 48(20):5713-5716. PMID: 3139281.

Behrens et al. 1987. Cancer Res. 47(2):414-418. PMID: 3539322.

Images

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A2780cis Cell Line. 24 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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