Anti-XRCC3 [XRCC3 10F1/6] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-XRCC3 [XRCC3 10F1/6] mAb
• Research fields: Genetics;Microbiology
• Clone: XRCC3 10F1/6
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 37.8 kDa
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: IHC ; WB
• Description: X-Ray Repair Cross Complementing 3 (XRCC3) is a RAD51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. XRCC3 is also a component of the homologous recombination pathway.
• Immunogen: His-tagged human XRCC3
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14
• Recommended controls: HeLa nuclear extracts.

Target Details

• Target: XRCC3
• Molecular weight: 37.8 kDa
• Tissue cell line specificity: HeLa nuclear extracts.
• Target background: X-Ray Repair Cross Complementing 3 (XRCC3) is a RAD51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. XRCC3 is also a component of the homologous recombination pathway.

Applications

• Application: IHC ; WB

Handling

• Format: Liquid
• Concentration: 0.94 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Agostini et al. 2015. Cancer Biol Ther. 16(8):1160-71. PMID: 26023803.
• Li et al. 2014. Sci Signal. 7(326):ra47. PMID: 24847116.
• Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
• Identification and purification of two distinct complexes containing the five RAD51 paralogs.
• Masson et al. 2001. Proc Natl Acad Sci U S A. 98(15):8440-6. PMID: 11459987.
• Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
• Tebbs et al. 1995. Proc Natl Acad Sci U S A. 92(14):6354-8. PMID: 7603995.
• Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene.