Cat. #151651
Anti-WIPI2 [2A2]
Cat. #: 151651
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: WIPI2
Class: Monoclonal
Application: IF ; FACS ; IF ; IP ; WB
Reactivity: Human ; Mouse
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sharon Tooze
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-WIPI2 [2A2]
- Research fields: Biochemistry;Cancer;Tissue-specific biology
- Clone: 2A2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 49 kDa
- Reactivity: Human ; Mouse
- Host: Mouse
- Application: IF ; FACS ; IF ; IP ; WB
- Description: This antibody is useful for the detection of WIPI-2, an essential protein for autophagy.
- Immunogen: Synthetic peptide corresponding to C-terminus of WIPI2b CSALRLDEDSEHPPMILRTD
- Isotype: IgG1 kappa
- Recommended controls: HEK293 cells
Target Details
- Target: WIPI2
- Molecular weight: 49 kDa
- Tissue cell line specificity: HEK293 cells
- Target background: WIPI2 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. WIPI2 is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. WIPI-2 is the mammalian homologue of Atg18.
Applications
- Application: IF ; FACS ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Nishimura et al. 2017. EMBO J. 36(12):1719-1735. PMID: 28495679.
- Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains.
- Martinez-Martin et al. 2017. Science. 355(6325):641-647. PMID: 28183981.
- A switch from canonical to noncanonical autophagy shapes B cell responses.
- Polson et al. 2010. Autophagy. 6(4):506-22. PMID: 20505359.
- Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
- Li et al. 2005. J Biol Chem. 280(29):26941-52. PMID: 15917248.
- Krppel-like factor-6 promotes preadipocyte differentiation through histone deacetylase 3-dependent repression of DLK1.
- Benzeno et al. 2004. Cancer Res. 64(11):3885-91. PMID: 15172998.
- Cyclin-dependent kinase inhibition by the KLF6 tumor suppressor protein through interaction with cyclin D1.
