Anti-TCP1 [22b]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-TCP1 [22b]
• Research fields: Apoptosis and autophagy;Cancer;Cell biology
• Clone: 22b
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Chicken ; Dog ; Hamster ; Mouse ; Rat
• Host: Rat
• Application: ELISA ; IP ; WB
• Description: TCP1, (a constituent of chaperone-containing TCP-1, CCT) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin.
• Immunogen: C-terminal half of the full length murine TCP protein
• Isotype: IgG2a
• Myeloma used: Y3.AG.1.2.3

Target Details

• Target: T-complex polypeptide-1 (TCP1; CCTalpha)
• Target background: TCP1, (a constituent of chaperone-containing TCP-1, CCT) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin.

Applications

• Application: ELISA ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Zhu H et al, 2010. Proteomics (19):3480-93 PMID 20815088
• Harrison-Lavoie et al. 1993. EMBO J. 12(7):2847-53. PMID: 8335000.
• A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins.
• Willison et al. 1989. Cell. 57(4):621-32. PMID: 2655925.
• The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes.