![Western blot using Anti-POT1; Polyclonal [MTA40] with siRNA KD of GFP (irrelevant RNA) and POT1.](https://cancertools.org/wp-content/uploads/485aae8b-0fba-4770-8f67-fa129905a0ea.jpg)
Cat. #151570
Anti-POT1 [MTA40]
Cat. #: 151570
Sub-type: Primary antibody
Unit size: 100 ug
Target: POT1
Class: Polyclonal
Application: IP ; WB
Reactivity: Human ; Mouse
Host: Rabbit
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Madalena Tarsounas
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-POT1 [MTA40]
- Research fields: Cancer;Genetics
- Clone: MTA40
- Tool sub type: Primary antibody
- Class: Polyclonal
- Conjugation: Unconjugated
- Molecular weight: 70 kDa
- Reactivity: Human ; Mouse
- Host: Rabbit
- Application: IP ; WB
- Description: POT1 (protection of telomeres 1) is part of the telomerase ribonucleotide (RNP) complex, which stabilises chromosomes by controlling telomere elongation. POT1 is a component of the double-stranded telomeric DNA-binding TRF1 complex which cis-inhibits telomerase. POT1 localization on telomeres diminishes in the absence of single-stranded DNA.
- Immunogen: Full-length human POT1 protein
- Recommended controls: U2OS cells
Target Details
- Target: POT1
- Molecular weight: 70 kDa
- Tissue cell line specificity: U2OS cells
- Target background: POT1 (protection of telomeres 1) is part of the telomerase ribonucleotide (RNP) complex, which stabilises chromosomes by controlling telomere elongation. POT1 is a component of the double-stranded telomeric DNA-binding TRF1 complex which cis-inhibits telomerase. POT1 localization on telomeres diminishes in the absence of single-stranded DNA.
Applications
- Application: IP ; WB
Handling
- Format: Liquid
- Concentration: 1.5 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS, 0.1% NaN3
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Martinez et al. 2010. Nat Cell Biol. 12(8):768-80. PMID: 20622869.
- Mammalian Rap1 controls telomere function and gene expression through binding to telomeric and extratelomeric sites.
- Martnez et al. 2009. Genes Dev. 23(17):2060-75. PMID: 19679647.
- Increased telomere fragility and fusions resulting from TRF1 deficiency lead to degenerative pathologies and increased cancer in mice.
