Cat. #151237
Anti-PIP2 [PIP2 2C11]
Cat. #: 151237
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Phosphatidylinositol biphosphate (PIP2)
Class: Monoclonal
Application: ChIP ; ELISA ; IHC ; IF ; IP ; Fn ; DB
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Giampietro Schiavo
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-PIP2 [PIP2 2C11]
- Research fields: Cell signaling and signal transduction;Neurobiology
- Clone: PIP2 2C11
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Host: Mouse
- Application: ChIP ; ELISA ; IHC ; IF ; IP ; Fn ; DB
- Description: PtdIns 4,5-P2 is a membrane phospholipid and an important component of signal transduction pathways.
- Immunogen: Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2.
- Isotype: IgM
- Myeloma used: Sp2/0-Ag14
- Recommended controls: To test the Ab efficiency use a lipid blot assay as described in References above.
Target Details
- Target: Phosphatidylinositol biphosphate (PIP2)
- Tissue cell line specificity: To test the Ab efficiency use a lipid blot assay as described in References above.
- Target background: PtdIns 4,5-P2 is a membrane phospholipid and an important component of signal transduction pathways.
Applications
- Application: ChIP ; ELISA ; IHC ; IF ; IP ; Fn ; DB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Toska et al. 2014. Oncogene. 33(43):5100-8. PMID: 24166496.
- Prohibitin is required for transcriptional repression by the WT1-BASP1 complex.
- Ozkucur et al. 2011. BMC Cell Biol. 12:4. PMID: 21255452.
- Persistent directional cell migration requires ion transport proteins as direction sensors and membrane potential differences in order to maintain directedness.
- Osborne et al. 2001. J Cell Sci. 114(Pt 13):2501-11. PMID: 11559758.
- Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing.
- Thomas et al. 1999. Biochem Soc Trans. 27(4):648-52. PMID: 10917659.
- Generation of phosphatidylinositol-specific antibodies and their characterization.
