#151440

Anti-Keratin6 [LHK6]

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Cat. #151440

Anti-Keratin6 [LHK6]

Cat. #: 151440

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Keratin 6

Class: Monoclonal

Application: ELISA ; FACS ; IHC ; IF ; WB

Reactivity: Human ; Mouse

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Nick Tidman

Institute: Queen Mary University of London

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Keratin6 [LHK6]
  • Alternate name: CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6EHuman Entrez Gene ID 3853-KRT6A, 3854-KRT6B, 286887-KRT6C Human SwissProt P2538-KRT6A, P4259-KRT6B, P48668-KRT6C Human Unigene 7779 Human Gene Symbol KRT6A; KRT6B; KRT6CHuman Chromosome Location 12q13.13; 12q13.13; 12q13.13
  • Research fields: Cell signaling and signal transduction
  • Clone: LHK6
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 55-57 kDa
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC ; IF ; WB
  • Description: LHK6 is a very good marker for hyperproliferative keratinocytes.
  • Immunogen: 11 amino aid sequence GSSTIKYTTTS of K6b C terminal
  • Isotype: IgG2a kappa
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Tonsil or basal cell carcinoma

Target Details

  • Target: Keratin 6
  • Molecular weight: 55-57 kDa
  • Tissue cell line specificity: Tonsil or basal cell carcinoma
  • Target background: Clone LHK6 recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratin 9 to 23) and on type II kerating (1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 6,16 and 17 are expressed in suprabasasl keratinocytes of wounded epidermis. Keratin 6 is found in acrosyringeal keratinocyes in normal skin.

Applications

  • Application: ELISA ; FACS ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Toivola et al. 2002. Mol Biol Cell. 13(6):1857-70. PMID: 12058054.
  • Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells.
  • Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
  • K15 expression implies lateral differentiation within stratified epithelial basal cells.
  • Machesney et al. 1998. Am J Pathol. 152(5):1133-41. PMID: 9588880.
  • Activated keratinocytes in the epidermis of hypertrophic scars.
  • Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
  • Basal cell-specific and hyperproliferation-related keratins in human breast cancer.