#151116

Anti-Keratin5 & 14 [LH8]

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Cat. #151116

Anti-Keratin5 & 14 [LH8]

Cat. #: 151116

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Keratin 5 & 14

Class: Monoclonal

Application: IHC ; IHC ; IF

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Irene Leigh

Institute: Queen Mary University of London

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Keratin5 & 14 [LH8]
  • Alternate name: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 1, KRT1Human Entrez Gene ID 3858 Human SwissProt P13645 Human Unigene 99936 Human Gene Symbol KRT1Human Chromosome Location 17q21.2
  • Research fields: Cell signaling and signal transduction
  • Clone: LH8
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Purpose: Marker
  • Conjugation: Unconjugated
  • Molecular weight: 56.5kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IHC ; IF
  • Description: LH8 may be useful as a basal cell marker and myoepithelial marker in stratified squamous or mixed epithelia.
  • Immunogen: The 50,000 Mr band (including K14) was cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
  • Isotype: IgM
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Extract of skin from psoriasis patient

Target Details

  • Target: Keratin 5 & 14
  • Molecular weight: 56.5kDa
  • Tissue cell line specificity: Mouse
  • Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 5 and 14 are the predominant keratins in basal layer keratinocytes in stratified epithelia. Their expression is restricted to basal cells and are found on all keratinocytes. Keratin 14 is one of two keratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Mutations in keratin 5 or 14 cause the skin blistering disorder epidermolysis bullosa simplex.

Applications

  • Application: IHC ; IHC ; IF

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Moser et al. 2014. PLoS One. 9(4):e94118. PMID: 24705787.
  • Prince et al. 2007. Proc Natl Acad Sci U S A. 104(3):973-8. PMID: 17210912.
  • Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma.
  • de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.
  • Keratin expression in discoid lupus erythematosus.