Anti-Keratin5 & 14 [LH8]

LH8 may be useful as a basal cell marker and myoepithelial marker in stratified squamous or mixed epithelia.

Contributors

Inventor Institute
Irene Leigh Queen Mary University of London
Cat. #: 151116
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Cell signaling and signal transduction
Application: IHC ; IHC ; IF
Target: Keratin 5 & 14
Reactivity: Human
Clone: LH8
Host: Mouse
Class: Monoclonal
Alternate name: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 1, KRT1Human Entrez Gene ID 3858 Human SwissProt P13645 Human Unigene 99936 Human Gene Symbol KRT1Human Chromosome Location 17q21.2
Product description: LH8 may be useful as a basal cell marker and myoepithelial marker in stratified squamous or mixed epithelia.
Conjugation: Unconjugated
Isotype: IgM
Molecular weight: 56.5kDa
Immunogen: The 50,000 Mr band (including K14) was cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
Myeloma used: Sp2/0-Ag14
Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 5 and 14 are the predominant keratins in basal layer keratinocytes in stratified epithelia. Their expression is restricted to basal cells and are found on all keratinocytes. Keratin 14 is one of two keratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Mutations in keratin 5 or 14 cause the skin blistering disorder epidermolysis bullosa simplex.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice
References:

Moser et al. 2014. PLoS One. 9(4):e94118. PMID: 24705787.

Prince et al. 2007. Proc Natl Acad Sci U S A. 104(3):973-8. PMID: 17210912.

Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma.

de Berker et al. 1995. Exp Dermatol. 4(6):350-6. PMID: 8608342.

Keratin expression in discoid lupus erythematosus.

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