Cat. #151439
Anti-Integrin a4 [BU49]
Cat. #: 151439
Sub-type: Primary antibody
Unit size: 100 ug
Target: Integrin alpha 4 (CD49d)
Class: Monoclonal
Application: FACS ; IP
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Roy Jefferis
Institute: University of Birmingham
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Integrin a4 [BU49]
- Alternate name: Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor, Alpha-2 Subunit; Human Platelet Alloantigen System 5; Platelet Glycoprotein GPIa; Platelet Antigen Br; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR
- Research fields: Cell biology;Cell signaling and signal transduction;Immunology;Stem cell biology;Tissue-specific biology
- Clone: BU49
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 150 kDa
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IP
- Description: BU49 induces phosphorylation of a cAMP response element-binding protein (CREB) in the human monocyte-like cell line, U937.
- Immunogen: Hicett myeloma cell line
- Isotype: IgG1
Target Details
- Target: Integrin alpha 4 (CD49d)
- Molecular weight: 150 kDa
- Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha 4 (CD49d) is a 150 kDa protein characterised by a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain. Hetrodimers are formed with integrin beta 1 and 7.
Applications
- Application: FACS ; IP
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Ikewaki et al. 2002. Microbiol Immunol. 46(10):685-95. PMID: 12477247.
- A very late activating antigen-alpha4 (CD49d) monoclonal antibody, BU49 induces phosphorylation of a cAMP response element-binding protein (CREB), resulting in induction of homotypic cell aggregation and enhancement of interleukin-8 (IL-8) production.
- Nojima et al. 1992. J Exp Med. 175(4):1045-53. PMID: 1372641.
- Ligation of VLA-4 on T cells stimulates tyrosine phosphorylation of a 105-kD protein.
