Cat. #161648
Anti-HLA-E [3D12]
Cat. #: 161648
Sub-type: Primary antibody
Target: HLA-E class I complexes
Class: Monoclonal
Application: FACS, IP, ELISA, Histology, No Western Blot.
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Daniel Geraghty
Institute: Fred Hutchinson Cancer Research Center
Primary Citation: lshitani et al. 2003. Journal of Immunology. 171(3):1376-84. PMID: 12874228
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-HLA-E [3D12]
- Research fields: Developmental biology;Immunology
- Clone: 3D12
- Tool sub type: Primary antibody
- Class: Monoclonal
- Molecular weight: ~40kDa
- Reactivity: Human
- Host: Mouse
- Application: FACS, IP, ELISA, Histology, No Western Blot.
- Description: MoAb was combined to examine HLA expression in placental tissues for all the known forms of the nonclassical HLA-E class I molecules that is important in the maternal immune response during pregnancy. Antibody 3D12 showed that HLA-E peptide binding was correlated with surface expression.
- Isotype: IgG1
Target Details
- Target: HLA-E class I complexes
- Molecular weight: ~40kDa
- Target background: Antibody is used to examine expression in placental tissues for all the known forms of the HLA-E nonclassical class I molecules.
Applications
- Application: FACS, IP, ELISA, Histology, No Western Blot.
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: The antibody solution should be stored undiluted between 2° C and 8° C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4° C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20° C or -80° C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
References
- lshitani et al. 2003. Journal of Immunology. 171(3):1376-84.PMID: 12874228
- Lee et al. 1998. J. Immunol.?160(10):4951-60. PMID: 9590243
