Anti-HLA-A2 [BB7.2]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-HLA-A2 [BB7.2]
• Research fields: Immunology
• Clone: BB7.2
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; Fn
• Description: Monoclonal antibody directed against HLA-A2 histocompatibility antigen, used to aid investigation into foreign antigen presentation and HLA mRNA production.
• Immunogen: Papain solubilised HLA-A2,
• Immunogen uniprot id: P04439
• Isotype: IgG2b
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Human Leukocyte Antigen- A2
• Target background: Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). The polymorphic HLA-A, -B, -C glycoproteins have a molecular weight of 43,000 daltons and are expressed on the surfaces of nearly all somatic cells, except red blood cells. This antibody can be used for HLA typing. Anti-HLA-A2 BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by the A.02 allele. It recognises an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands. Anti-HLA-A2 BB7.2 binds to an epitope at the C-terminus of the alpha-2 helix within the human HLA-A2 histocompatibility antigen. This antibody has exhibited blocking activity. Anti-HLA monoclonal antibodies can help identify in vitro translation production from HLA region mRNA, alongside isolating specific mRNA via precipitation or binding of polysomes. Anti-HLA-A2 can aid study within the immune response, including the presentation of foreign antigens to the immune system.

Applications

• Application: FACS ; IHC ; IF ; Fn

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

References

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