Anti-CD29 [12G10]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD29 [12G10]
• Alternate name: Integrin Subunit Beta 1; Glycoprotein Iia; MSK12; GPIIA; FNRB; MDF2; Very Late Activation Protein; Beta Polypeptide; Fibronectin Receptor Subunit Beta; Integrin VLA-4 Beta Subunit; CD29 Antigen; VLA-BETA; CD29; VLAB
• Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Genetics;Immunology
• Clone: 12G10
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
• Description: CD29 is a member of the family of integrins, membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells.
• Immunogen: Purified human ?1 integrin preparation from HT1080 fibrosarcoma cell extract.
• Isotype: IgG1
• Myeloma used: P3X63Ag8.653

Target Details

• Target: CD29 (Integrin b1 chain)
• Target background: CD29 is a member of the family of integrins, membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells.

Applications

• Application: ELISA ; FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Chen et al. 2009. PLoS One. 4(8):e6565. PMID: 19668337.
• MMP7 shedding of syndecan-1 facilitates re-epithelialization by affecting alpha(2)beta(1) integrin activation.
• Yamamoto et al. 2009. Endocrinology. 150(2):990-9. PMID: 18845630.
• N-acetylglucosaminyltransferase V regulates extravillous trophoblast invasion through glycosylation of alpha5beta1 integrin.
• Yamamoto et al. 2007. Br J Cancer. 97(11):1538-44. PMID: 17971775.
• Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis.
• Cavallo-Medved et al. 2005. J Cell Sci. 118(Pt 7):1493-503. PMID: 15769846.
• Caveolin-1 mediates the expression and localization of cathepsin B, pro-urokinase plasminogen activator and their cell-surface receptors in human colorectal carcinoma cells.
• Avizienyte et al. 2002. Nat Cell Biol. 4(8):632-8. PMID: 12134161.
• Src-induced de-regulation of E-cadherin in colon cancer cells requires integrin signalling.
• Iba et al. 2000. J Cell Biol. 149(5):1143-56. PMID: 10831617.
• The cysteine-rich domain of human ADAM 12 supports cell adhesion through syndecans and triggers signaling events that lead to beta1 integrin-dependent cell spreading.
• Mould et al. 1998. Biochem J. 331 ( Pt 3):821-8. PMID: 9560310.
• Regulation of integrin function: evidence that bivalent-cation-induced conformational changes lead to the unmasking of ligand-binding sites within integrin alpha5 beta1.
• Mould et al. 1995. Biochem Soc Trans. 23(3):395S. PMID: 8566283.
• Regulation of integrin alpha 5 beta 1 function by anti-integrin antibodies and divalent cations.
• Mould et al. 1995. FEBS Lett. 363(1-2):118-22. PMID: 7537221.
• Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit.