Cat. #151848
Anti-BORIS [4A7]
Cat. #: 151848
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: BORIS
Class: Monoclonal
Application: ChIP ; ELISA ; IF ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Elena Klenova
Institute: University of Essex
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-BORIS [4A7]
- Alternate name: Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC binding factor (zinc finger protein) like, CCCTC-binding factor, CT27, CTCF paralog, CTCF T, CTCF-like protein, Ctcfl, CTCFL_HUMAN, dJ579F2.2, HMG 1L1, HMGB1L1, MGC163358, MGC16915, MGC16916
- Research fields: Cancer;Genetics
- Clone: 4A7
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 83 kDa
- Reactivity: Human
- Host: Mouse
- Application: ChIP ; ELISA ; IF ; IP ; WB
- Description: Monoclonal antibody to understand the role of BORIS protein in prostate cancers.
- Immunogen: The N-terminal domain (aa 2-257) of human BORIS (CTCFL) protein, expressed in E.coli
- Immunogen uniprot id: Q8NI51
- Isotype: IgM kappa
- Recommended controls: Testis
Target Details
- Target: BORIS
- Molecular weight: 83 kDa
- Tissue cell line specificity: Testis
- Target background: BORIS protein is a transcriptional repressor encoded by CTCFL gene, and is normally present at high levels in testis. BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50). BORIS protein is detected in all prostate cell lines and prostate tumours, but it is absent in benign prostatic hyperplasia tissues. Increased BORIS protein levels correlate with higher Gleason scores, T-stage and androgen receptor (AR) protein levels in prostate tumours. This reagent is for research and as a diagnostic tool.
Applications
- Application: ChIP ; ELISA ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-BORIS [20B11]
References
- Cheema et al. 2014. Prostate. 74(2):164-76. PMID: 24123052.
- Expression of the cancer-testis antigen BORIS correlates with prostate cancer.
