Cat. #152092
Anti-APC [c-APC 28.9]
Cat. #: 152092
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: APC
Class: Monoclonal
Application: IHC ; IF ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-APC [c-APC 28.9]
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Colorectal
- Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Genetics;Neurobiology;Stem cell biology;Tissue-specific biology
- Clone: c-APC 28.9
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 320 kDa
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IF ; IP ; WB
- Description: C-APC 28.8 can be used for APC expression and detection of APC mutations.
- Immunogen: human APC C-terminal fusion with MBP (maltose binding protein)
- Isotype: IgG1 kappa
- Recommended controls: Colon cell line HCT116
Target Details
- Target: APC
- Molecular weight: 320 kDa
- Tissue cell line specificity: Colon cell line HCT116
- Target background: The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Clone c-APC 28.9 is useful for the detection of APC expression and detection of mutations. This antibody has been used routinely for Western blotting on colon cell line HCT116 extract which expresses full-length APC.
Applications
- Application: IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Kariola et al. 2005. Fam Cancer. 4(2):187-90. PMID: 15951972.
- APC and beta-catenin protein expression patterns in HNPCC-related endometrial and colorectal cancers.
- Fodde et al. 2001. Nat Cell Biol. 3(4):433-8. PMID: 11283620.
- Mutations in the APC tumour suppressor gene cause chromosomal instability.
