#154111

Human Melanocyte [bFGF] Cell Line

Cat. #154111

Human Melanocyte [bFGF] Cell Line

Cat. #: 154111

Sub-type: Primary

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Skin

Model: Primary line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Pranab K Das

Tool Details
Handling

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Human Melanocyte [bFGF] Cell Line
  • Research fields: Cancer;Cell biology
  • Tool sub type: Primary
  • Parental cell: Human foreskin
  • Organism: Human
  • Tissue: Skin
  • Model: Primary line
  • Conditional: No
  • Description: Melanocytes are the cell of origin for a number of conditions including melanoma an aggressive type of skin cancer and vitiligo a long-term condition characterised by patches of the skin losing their pigment. Melanoma is the most dangerous type of skin cancer. Globally, in 2012, it newly occurred in 232,000 people. In 2015 there were 3.1 million with active disease which resulted in 59,800 deaths. Australia and New Zealand have the highest rates of melanoma in the world. While Vitiligo affects 1% of the worlds population with no known cause. Primary melanocytes can be used as a tool for research into different melanocytic lineages
  • Production details: Melanocyte cultures were established from neonatal foreskin using standard methods. Culture medium was supplemented with bFGF.
  • Biosafety level: 1

Handling

  • Format: Frozen
  • Growth medium: Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 100ng/ml bFGF, 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100g/ml streptomycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes