phOC-iNOS Vector

This plasmid increases iNOS protein and total nitrite levels in PC3 and DU145 cells, but not LNCaP orHT29 (McCarthy et al., 2007).

Contributors

Inventor Institute
Helen McCarthy Queen's University Belfast
Cat. #: 153288
Tool sub type: pcDNA 3.1
Research Fields: Cancer;Metabolism;Neurobiology
Target: Inducible Nitric Oxide Synthase
Bacterial resistance: Kanamycin
Alternate name: Nitric Oxide Synthase
Product description: phOC-iNOS Plasmid is a human nitric oxide synthase (iNOS) transgene driven by a human osteocalcin (hOC) promoter to control inducible nitric oxide synthase (iNOS) transgene expression in hormone refractory prostate cancer (HRPC). The plasmid was originally developed as an anti-cancer therapeutic with a view to a gene therapy strategies. Studies in the literature have demonstrated that this plasmid increases iNOS protein and total nitrite levels in PC3 and DU145 cells, but not LNCaP or HT29 (McCarthy et al., 2007). The plasmid serves as a useful tool in cardiovascular studies or any physiology associated with vasodilation.
Application notes: Recommended for transpormation in DH5 alpha bacterial cells iNOS gene is 4.4kb
Shipping conditions: Dry Ice
References:

McCarthy et al. 2007. J Gene Med. 9(6):511-20. PMID: 17471586.

Human osteocalcin: a strong promoter for nitric oxide synthase gene therapy, with specificity for hormone refractory prostate cancer.

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