pASS2SUL vector

Protein Purification; Protein Biochemistry

Contributors

Inventor Institute
Dr. Patrick Loll Drexel University
Cat. #: 156510
Tool sub type: pASK
Research Fields: Other
Target: Strep-II tagged small ubiquitin related modifier (SUMO) fusion target protein
Backbone size without insert: 3476
Bacterial resistance: Ampicillin
Product description: Protein Purification; Protein Biochemistry
Additional notes: The pASS2SUL vector is part of a set of ligation independent cloning SUMO fusion vectors that allow for recombinant proteins to be produced with any desired sequence at the amino terminus, so long as the initial residue is not proline. This set of vectors generates affinity tagged SUMO fusion target proteins that allow the target protein to be expressed in <i> E.coli </i> and purified using subtractive affinity chromatography. The purified SUMO fusion is treated with recombinant SUMO protease (generated by pdtUD1 vector) to yield the mature target protein. The pASS2SUL vector yields His6 tagged SUMO fusion proteins. Recombinant SUMO protease cleaves His6 SUMO from the purified target protein to yield the final mature protein. The pASS2SUL vector was constructed base on the pASK vector architecture. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Related product: pdtUD1 vector
Application notes: Protein Purification; Protein Biochemistry
References:

Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035.

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