Cat. #153458
pRSF-1b-UbcH5B Vector
Cat. #: 153458
Sub-type: pRSF-1b
Availability: 3-5 days
Target: UbcH5B
Bacterial Resistance: Kanamycin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Danny Huang
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: pRSF-1b-UbcH5B Vector
- Alternate name: UBE2D2, E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B, ubiquitin conjugating enzyme E2 D2
- Tool sub type: pRSF-1b
- Backbone size without insert: 3669
- Bacterial resistance: Kanamycin
- Description: Protein modification through ubiquitination is an important cellular mechanism to target abnormal or short-lived proteins for degradation. The process of ubiquitination involves three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes and ubiquitin-protein ligases. UbcH5B, encoded by the UBE2D2 gene, is a member of the E2 ubiquitin-conjugating enzyme family. UbcH5B is involved in the ubiquitination of p53 and two alternatively spliced transcript variants have been discovered which encode distinct isoforms.
Handling
- Storage conditions: -20° C
- Shipping conditions: Dry Ice
Target Details
- Target: UbcH5B
Related Tools
- Related tools: pGEX4T1-CBL-B Vector
References
- Dou et al. 2012. Nat Struct Mol Biol. 19(9):876-83. PMID: 22902369.
- BIRC7-E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer.
