Endo-Beta-N-acetylglucosaminidase (Endo Tv), Enzyme

This fungal endo-glycosidase (ENGase) from Trichoderma viride will release “Man5GlcNAc to Man9GlcNAc oligosaccharides from RNase B, ManGlcNAc, Man3GlcNAc, Man5GlcNAc, Man6GlcNAc and Man8GlcNAc from ovalbumin, and high-mannose and oligomannose structures from yeast invertase.”1 However, this enzyme will “not act on fucosylated, hybrid, complex-type or bisecting N-acetyl-glucosamine-containing N-linked oligosaccharide structures from ovalbumin, IgG and fetuin.”1 The exclusive […]

Contributors

Inventor Institute
Jared Gerlach National University of Ireland Galway
Cat. #: 153542
Research Fields: Metabolism
Product description: This fungal endo-glycosidase (ENGase) from Trichoderma viride will release “Man5GlcNAc to Man9GlcNAc oligosaccharides from RNase B, ManGlcNAc, Man3GlcNAc, Man5GlcNAc, Man6GlcNAc and Man8GlcNAc from ovalbumin, and high-mannose and oligomannose structures from yeast invertase.”<sup>1</sup> However, this enzyme will “not act on fucosylated, hybrid, complex-type or bisecting N-acetyl-glucosamine-containing N-linked oligosaccharide structures from ovalbumin, IgG and fetuin.”<sup>1</sup> The exclusive preference of this enzyme “for high mannose type oligosaccharides without substitutions is in contrast to the bacterial ENGases, Endo H and Endo F1, which additionally cleave hybrid structures, and also tolerate fucosylation in the case of Endo H.”<sup>1</sup> This enzyme has an ~Mr of 35 kDa as determined by SDS-PAGE. 1) Gerlach et al, Molecular BioSystems. 2012;8(5):1472-1481 • Cleaves within the chitobiose core of high- and oligomannose-type and N-linked oligosaccharides commonly found on a variety of glycoproteins • Can be used in preparative and analytical deglycosylation protocols and other applications involving high-mannose containing glycoproteins The use of Endo Tv in an analysis of the surface glycosylation of urinary extracellular vesicles is described in Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD. Surface glycosylation profiles of urine extracellular vesicles. PloS One. 2013 Sep 19;8(9):e74801.
Storage conditions: 4° C
References:

Gerlach et al. 2012. Mol. BioSyst. 8:1472-1481 PMID: 22373601

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