Cat. #153757
Alox12bf/fK14CreERT2 Mouse
Cat. #: 153757
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Ichthyosis
Model: Conditional KO
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Contributor
Inventor: Peter Krieg
Institute: Deutsches Krebsforschungszentrum (DKFZ) German Cancer Research Center
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Alox12bf/fK14CreERT2 Mouse
- Alternate name: Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase, Epidermis-type lipoxygenase 12. UniProt ID:O75342
- Research fields: Cell biology;Drug development;Genetics;Metabolism
- Tool sub type: Mouse
- Disease: Ichthyosis
- Model: Conditional KO
- Conditional: Yes
- Conditional description: Tamoxifen inducible ablation of arachidonate 12-lipoxygenase (Alox12b) in epidermis (and other K14 expressing tissues) via conditional Cre-ERT2 expression under the K14 promoter.
- Description: Tamoxifen inducible ablation of arachidonate 12-lipoxygenase (Alox12b) in epidermis (and other K14 expressing tissues). Alox12b plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. Ichthyosis, congenital, autosomal recessive 2 (ARCI2). Ichthyosis is a family of rare genetic skin disorders characterised by dry, thickened, scaly skin.
- Genetic background: SKH1
- Additional notes: Genetic modifications: a) Insertion of loxP sites flanking essential exon 8 of Alox12b (=floxed Alox12b)(Epp et al., JBC 2007) b) K14 cre/ESR1T2 (knock in) (Amen et al. Hum Mol Genet 2013) c) Crossing of the floxed Alox12b mice with the K14Cre mice (unpublished) d) Cross-bred with SKH1 hairless mice (unpublished) Mouse model also known as: Short name: SKH1/Alox12bflox/K14CreJ Full name: STOCK-Hrhr Alox12btm1Krie Krt14tm1.1(cre/ESR1T2)#Dkfz / Krie
Target Details
- Target: Arachidonate 12-lipoxygenase (ALOX12B)
Related Tools
- Related tools: Anti-12R-LOX, Polyclonal
References
- Epp, N., Fürstenberger, G., Müller, K., de Juanes, S., Leitges, K., Hausser, I., Thieme, F. Liebisch, G., Schmitz, G., and Krieg, P. 12R-lipoxygenase deficiency disrupts epidermal barrier function. J. Cell. Biol. 177, 173-182, 2007. Krieg, P., Fürstenberger, G. The role of lipoxygenases in epidermis. Biochim Biophys Acta. 1841, 390-400, 2014. Amen, N, Mathow D, Rabionet M, Sandhoff R, Langbein L, Gretz N, Jäckel C, Gröne HJ, Jennemann R. Differentiation of epidermal keratinocytes is dependent on glucosylceramide:ceramide processing. Hum Mol Genet. 2013 Oct 15
- 22(20):4164-79. doi: 10.1093/hmg/ddt264. Epub 2013 Jun 6.
