SW480 EGFP-lamin A cell line clone 2bb3

Duke’s stage C/Broders’ grade 3-4 colorectal cancer cell line stably transfected with EGFP tagged lamin A.

Contributors

Inventor Institute
Stefan Przyborski University of Durham
Cat. #: 162199
Organism: Human
Tissue: Colon
Model: Genetically modified cell line
Morphology: Epithelial
Growth properties: Adherent
Alternate name: SW480 GFP-lamin A clone 12
Product description: Colon cancer cell line with low endogenous lamin A protein expression, rescued by stable transfection of EGFP-lamin A. Suitable for studying the effect of the presence of lamin A on cancer cell properties.
Receptors of note: No
Parental cell line: SW480
Initial handling information: Continue to store at ?-70deg C. To bring up cells, defrost quickly in 37?C water bath and seed in petri dish/flask. Once cells have adhered to culture surface, change media to DMSO-free media.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 100units/ml penicillin / 100ug/ml streptomycin
Subculture routine: Passage at 70-80% confluencey in the presence of 0.25% trypsin in 0.5mM EDTA/PBS. Incubate at 37?C, 5% Co2, humidified for 2 minutes. Split 1:4.
Atmosphere: Without CO2
Storage medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 10% DMSO
Recommended controls: Same cell line stably transfected with EGFP not tagged to any other DNA
References:

Unpublished

Related Tools for Cell lines

Cat. # Tool Name
151447 CMT 64 Cell Line Key Info View Tool
151484 2fTGH Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool
151576 MEF RAF1 KO Cell Line Key Info View Tool
151631 C-Raf KI D486A Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.