Cat. #161920
SK-MEL-5 Cell Line
Cat. #: 161920
Organism: Human
Tissue: Skin
Model: Tumourigenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Toshitada Takahashi
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-MEL-5 Cell Line
- Cancer: Skin cancer
- Organism: Human
- Gender: Female
- Tissue: Skin
- Donor: This cell line was established in 1974 from a metastatic site (axillary lymph node) in a 24-year-old Caucasian female with malignant melanoma.
- Morphology: Stellate
- Growth properties: Adherent
- Model: Tumourigenic
- Model description: Tumourigenic in nude mice; forms malignant melanoma
- Products or characteristics of interest: Karyotype: modal number = 120; range = 95 to 133. This is a hyperpentaploid human cell line with the modal chromosome number of 120, occurring in 9% of cells. The majority of cells had chromosome counts of 114-123. Among 14 marker chromosomes that were common to most cells, generally 9q+ had four copies per cell, t(1q8q) and del(5) (q34) had three copies per cell, and 14q+ had two copies per cell. Numerous others were also found in single copies, all infrequently. Three normal X chromosomes were found in most cells, N9 was absent, and N7 and N20 had seven to eight copies per cell. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; PGM1, 1-2; PGM3, 1
- Description: SK-MEL-5 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type O; Rh+; HLA A2, A11, B40, Bw16
Applications
- Application: 3D cell culture
Handling
- Growth medium: EMEM supplemented with FBS to a final concentration of 10%
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium, 95%; DMSO, 5%
- Storage conditions: Vapor phase of liquid nitrogen
- Str profiling: D3S1358: 16,17TH01: 6,9D21S11: 29D18S51: 15,16Penta_E: 5,12D5S818: 11,13D13S317: 10,12D7S820: 9,12D16S539: 10,12CSF1PO: 10,13Penta_D: 9,11Amelogenin: XvWA: 14,18D8S1179: 12,15TPOX: 11FGA: 20.2,22D19S433: 14,15D2S1338: 17,25
References
- Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.
