Cat. #161911
SK-BR-3 Cell Line
Cat. #: 161911
Availability: 8-10 weeks
Organism: Human
Tissue: Breast; Mammary gland
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Germain Trempe
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-BR-3 Cell Line
- Cancer: Adenocarcinoma;Breast cancer
- Organism: Human
- Gender: Male
- Tissue: Breast; Mammary gland
- Donor: 43-year-old, White, female adenocarcinoma patient with blood type A+ who had been treated with radiation, steroids, cytoxan, and 5-fluorouracil
- Morphology: Epithelial; Ultrastructural features include microvilli and desmosomes, glycogen granules, large lysosomes, bundles of cytoplasmic fibrils.
- Growth properties: Adherent
- Description: SK-BR-3 is a human breast cancer cell line that overexpresses the Her2 (Neu/ErbB-2) gene product. These cells display an epithelial morphology in tissue culture and are capable of forming poorly differentiated tumours in immunocompromised mice. The SK-BR-3 cells and products derived from it are used often as positive controls in assays for Her2. In addition, the cell line is also a useful preclinical model to screen for therapeutic agents targeting Her2 and to delineate mechanisms of resistance to Her2-targeted therapies.
- Application: 3D cell culture
- Biosafety level: 1
Applications
- Application: 3D cell culture
Handling
- Growth medium: McCoy's 5a Medium Modified supplemented with 10% FBS
- Temperature: 37° C
- Atmosphere: 95% Air, 5% CO2
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium, 95%; DMSO, 5%
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
- Str profiling: Amelogenin: X;CSF1PO: 12;D13S317: 11,12;D16S539: 9;D5S818: 9,12;D7S820: 9,12;TH01: 8,9;TPOX: 8,11;vWA: 17;D3S1358: 17;D21S11: 30,30.2;D18S51: 10,13;Penta_E: 10,11;Penta_D: 9,12;D8S1179: 12;FGA: 20;D19S433: 14;D2S1338: 20,25
References
- Fogh J et al. 1977. Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
