#153464

Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]

Please Enter Your Email ID
Email ID

Cat. #153464

Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]

Cat. #: 153464

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Eye

Model: Reporter

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jonathon Pines

Institute: University of Cambridge

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]
  • Alternate name: CCNB1
  • Research fields: Cell biology
  • Parental cell: RPE1
  • Organism: Human
  • Tissue: Eye
  • Model: Reporter
  • Conditional: Yes
  • Description: Somatic knock-in reporter cell with endogenous expression of cyclin B1 fused to the yellow fluorescent protein Venus (cyclin B1-Venus fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This Cyclin B1 cell line functions as a cell cycle reporter across the G2/mitotic-specific phase of the cell cycle. See also the Mad2 and Cyclin A2 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for cyclin B1 levels and activity.
  • Production details: To generate the cell line recombinant adenovirus-associated virus (rAAV)-mediated gene targeting was used to fuse the open reading frame (ORF) of yellow fluorescent protein (Venus) with exon 9 of the CCNB1 (Cyclin B1) gene in hTert-RPE1 cells (RPE1; retinal pigment epithelial). RPE1 cells were chosen because they have a normal diploid karyotype, are not transformed and exhibit little cell death when arrested in mitosis; tagging the endogenous Cyclin B1 protein in RPE1 cells avoided the compl...
  • Biosafety level: 1

Target Details

  • Target: Cyclin B1

Handling

  • Format: Frozen
  • Growth medium: F12/DMEM (Sigma: D6241) + 2mM GlutaMAX?„?‚ƒ?‚¤(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37oC; 5% CO2.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6] ; Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1] ; Ruby-Mad2 Reporter Cell Line

References

  • Collin et al., 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242