Cat. #154104
Piebaldism Melanocyte Cell Line
Cat. #: 154104
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Disease: Piebaldism
Model: Primary line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pranab K Das
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Piebaldism Melanocyte Cell Line
- Research fields: Cell biology;Immunology
- Parental cell: Lesional skin of piebaldism patient
- Organism: Human
- Disease: Piebaldism
- Model: Primary line
- Conditional: No
- Description: PiebaldismÄÂ? is a rareÄÂ? autosomal dominantÄÂ? disorder ofÄÂ? melanocyteÄÂ? development. Common characteristics include a congenital whiteÄÂ? forelock, scattered normal pigmented and hypo pigmentedÄÂ? maculesÄÂ? and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
- Production details: Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Handling
- Format: Frozen
- Growth medium: HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
