Piebaldism Melanocyte Cell Line

PiebaldismÂ?‚ is a rareÂ?‚ autosomal dominantÂ?‚ disorder ofÂ?‚ melanocyteÂ?‚ development. Common characteristics include a congenital whiteÂ?‚ forelock, scattered normal pigmented and hypo pigmentedÂ?‚ maculesÂ?‚ and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung’s disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool […]

Contributors

Inventor
Pranab K Das
Cat. #: 154104
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Immunology
Organism: Human
Model: Primary line
Product description: PiebaldismÂ?‚ is a rareÂ?‚ autosomal dominantÂ?‚ disorder ofÂ?‚ melanocyteÂ?‚ development. Common characteristics include a congenital whiteÂ?‚ forelock, scattered normal pigmented and hypo pigmentedÂ?‚ maculesÂ?‚ and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
Conditional: No
Production details: Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10Â?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100Â?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Parental cell line: Lesional skin of piebaldism patient
disease: Piebaldism
Format: Frozen
Shipping conditions: Dry ice
Growth medium: HAMs F10 supplemented with 10 ?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)

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