#154104

Piebaldism Melanocyte Cell Line

Please Enter Your Email ID
Email ID

Cat. #154104

Piebaldism Melanocyte Cell Line

Cat. #: 154104

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Disease: Piebaldism

Model: Primary line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Pranab K Das

Tool Details
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Piebaldism Melanocyte Cell Line
  • Research fields: Cell biology;Immunology
  • Parental cell: Lesional skin of piebaldism patient
  • Organism: Human
  • Disease: Piebaldism
  • Model: Primary line
  • Conditional: No
  • Description: PiebaldismÂ?‚ is a rareÂ?‚ autosomal dominantÂ?‚ disorder ofÂ?‚ melanocyteÂ?‚ development. Common characteristics include a congenital whiteÂ?‚ forelock, scattered normal pigmented and hypo pigmentedÂ?‚ maculesÂ?‚ and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
  • Production details: Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10Â?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100Â?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).

Handling

  • Format: Frozen
  • Growth medium: HAMs F10 supplemented with 10 ?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice