Cat. #151673
PEO4 Cell Line
Cat. #: 151673
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Ovary
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Simon Langdon
Institute: Cancer Research UK Edinburgh Centre
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: PEO4 Cell Line
- Alternate name: PE04; PEO-4
- Cancer: Adenocarcinoma
- Cancers detailed: Adenocarcinoma
- Research fields: Cancer;Drug development
- Organism: Human
- Tissue: Ovary
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: The PEO4 cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists. PEO4 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO4 was collected after clinical resistance developed to chemotherapy. PEO4 is tumourigenic in immunologically-deprived CBA mice. PEO4 exhibits good growth in semi-solid medium (agar). PEO4 is from the same patient as the PEO1 and PEO6 cell lines.
- Cellosaurus id: CVCL_2690
Handling
- Format: Frozen
- Growth medium: RPMI 1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 36 hours. Cultures should be media changed every 2-3 days if not splitting.
Related Tools
- Related tools: PEO6 Cell Line ; PEO14 Cell Line ; PEO16 Cell Line ; PEO23 Cell Line ; PEO1-CDDP Cell Line ; TO14 Cell Line ; PEA1 Cell Line ; PEA2 Cell Line
References
- Matassa et al. 2016. Cell Death Differ. :. PMID: 27206315.
- Oxidative metabolism drives inflammation-induced platinum resistance in human ovarian cancer.
- The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer.
- Huang et al. 2015. Oncotarget. :. PMID: 26683361.
- Shields et al. 2015. Mol Syst Biol. 11(12):842. PMID: 26655797.
- A genome-scale screen reveals context-dependent ovarian cancer sensitivity to miRNA overexpression.
- Patel et al. 2015. Cell Onc...
