Cat. #151673
PEO4 Cell Line
Cat. #: 151673
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Ovary
Disease: Cancer
Model: Tumour line
£598.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Simon Langdon
Institute: Cancer Research UK Edinburgh Centre
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: PEO4 Cell Line
- Alternate name: PE04; PEO-4
- Cancer type: Ovarian cancer
- Cancers detailed: High-Grade Serous Ovarian (HGSOC)
- Research fields: Cancer;Drug development
- Organism: Human
- Tissue: Ovary
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: No
- Description: The PEO4 cell line is one of nine in the PE ovarian cancer panel (derived from 4 patients at varying stages of disease, isolated from various malignant sites and treatment timepoints), providing a model system for ovarian cancer biology and therapeutic response. PEO4 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with high-grade serous ovarian carcinoma (historically described as serous adenocarcinoma). The patient had previously received cisplatin, 5-fluorouracil and chlorambucil treatment, and PEO4 was established following the development of clinical resistance to chemotherapy. PEO4 is tumourigenic in immunologically deprived CBA mice and represents an acquired platinum-resistant model within the PEO cell line series used to study treatment-driven tumour evolution. PEO4 exhibits good growth in semi-solid medium (agar) and is from the same patient as the PEO1 and PEO6 cell lines.
- Biosafety level: 1
- Cellosaurus id: CVCL_2690
Handling
- Format: Frozen
- Growth medium: RPMI 1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 36 hours. Cultures should be media changed every 2-3 days if not splitting.
Related Tools
- Related tools: PEO6 Cell Line ; PEO14 Cell Line ; PEO16 Cell Line ; PEO23 Cell Line ; PEO1-CDDP Cell Line ; TO14 Cell Line ; PEA1 Cell Line ; PEA2 Cell Line
References
- Matassa et al. 2016. Cell Death Differ. PMID: 27206315.
- Huang et al. 2015. Oncotarget. PMID: 26683361.
- Shields et al. 2015. Mol Syst Biol. 11(12):842. PMID: 26655797.
- Patel et al. 2015. Cell Oncol (Dordr). PMID: 26266765.
- Hearn et al. 2015. Proc Natl Acad Sci U S A. :. PMID: 26162681.
- Tuthill et al. 2015. Oncogene. 34(16):2138-44. PMID: 24909167.
- Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737.
- Langdon et al. 1988. Cancer Res. 48(21):6161-5. PMID: 3167862.
- Langdon et al. 1988. Cancer Res. 48(21):6166-72. PMID: 3167863.
