#151784

PEO1-CDDP Cell Line

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Cat. #151784

PEO1-CDDP Cell Line

Cat. #: 151784

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Ovary

Disease: Cancer

Model: Cisplatin Resistance

£575.00

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Contributor

Inventor: Simon Langdon

Institute: Cancer Research UK Edinburgh Centre

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: PEO1-CDDP Cell Line
  • Cancer: Adenocarcinoma
  • Cancers detailed: Adenocarcinoma
  • Research fields: Cancer;Cell signaling and signal transduction;Drug development
  • Tool sub type: Continuous
  • Parental cell: PEO1
  • Organism: Human
  • Tissue: Ovary
  • Disease: Cancer
  • Model: Cisplatin Resistance
  • Description:  The PEO1-CDDP Cell Line is an in-vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance. Coupled with this change in drug sensitivity, this model also has a modified responsiveness to ligands of the erbB receptor family. This model will be helpful in studying the changes in cellular signalling after drug treatment, which are currently attracting a great deal of interest and this model will be of particular relevance when cytotoxic therapy is combined with signalling inhibitors.
  • Production details: The PE01CDDP cell line was established by in vitro exposure of the PE01 line to cisplatin commencing at 25 nmol/L cisplatin and increasing to 1 Amol/L over a period of 4 months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further 4 months to ensure that the resistance remained stable. PEO1 CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines (ECACC Catalogue numbers 10032308, 10032309 & 10032310).
  • Cellosaurus id: CVCL_Y032

Target Details

  • Target: Human ovarian cancer; oestrogen receptor, drug resistance, cisplatin resistance

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 containing 10% heat-inactivated FCS, penicillin (100 units/mL), and streptomycin (100 ug/mL). Cells were maintained routinely at 37°C in a humidified atmosphere of 5% CO2 in air.
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: PEO1 Cell Line ; PEO4 Cell Line ; PEO6 Cell Line ; PEO14 Cell Line ; PEO16 Cell Line ; PEO23 Cell Line ; PEA1 Cell Line ; PEA2 Cell Line ; TO14 Cell Line

References

  • Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.
  • Altered ErbB receptor signaling and gene expression in cisplatin-resistant ovarian cancer.