Cat. #151784
PEO1-CDDP Cell Line
Cat. #: 151784
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Ovary
Disease: Cancer
Model: Cisplatin Resistance
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Simon Langdon
Institute: Cancer Research UK Edinburgh Centre
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: PEO1-CDDP Cell Line
- Cancer: Adenocarcinoma
- Cancers detailed: Adenocarcinoma
- Research fields: Cancer;Cell signaling and signal transduction;Drug development
- Tool sub type: Continuous
- Parental cell: PEO1
- Organism: Human
- Tissue: Ovary
- Disease: Cancer
- Model: Cisplatin Resistance
- Description: The PEO1-CDDP Cell Line is an in-vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance. Coupled with this change in drug sensitivity, this model also has a modified responsiveness to ligands of the erbB receptor family. This model will be helpful in studying the changes in cellular signalling after drug treatment, which are currently attracting a great deal of interest and this model will be of particular relevance when cytotoxic therapy is combined with signalling inhibitors.
- Production details: The PE01CDDP cell line was established by in vitro exposure of the PE01 line to cisplatin commencing at 25 nmol/L cisplatin and increasing to 1 Amol/L over a period of 4 months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further 4 months to ensure that the resistance remained stable. PEO1 CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines (ECACC Catalogue numbers 10032308, 10032309 & 10032310).
- Cellosaurus id: CVCL_Y032
Target Details
- Target: Human ovarian cancer; oestrogen receptor, drug resistance, cisplatin resistance
Handling
- Format: Frozen
- Growth medium: RPMI 1640 containing 10% heat-inactivated FCS, penicillin (100 units/mL), and streptomycin (100 ug/mL). Cells were maintained routinely at 37°C in a humidified atmosphere of 5% CO2 in air.
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: PEO1 Cell Line ; PEO4 Cell Line ; PEO6 Cell Line ; PEO14 Cell Line ; PEO16 Cell Line ; PEO23 Cell Line ; PEA1 Cell Line ; PEA2 Cell Line ; TO14 Cell Line
References
- Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.
- Altered ErbB receptor signaling and gene expression in cisplatin-resistant ovarian cancer.
