PEO1-CDDP Cell Line

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Cisplatin-resistant PEO1 derivative for studying acquired platinum resistance in a BRCA2-mutant ovarian cancer background

Contributors

Inventor Institute
Simon Langdon Cancer Research UK Edinburgh Centre
SKU: 151784
Product description: PEO1-CDDP is a cisplatin-resistant derivative of PEO1, selected through repeated cisplatin exposure and subsequently maintained without drug to confirm that the resistant phenotype was stable. PEO1 itself is a BRCA2-mutant, platinum-sensitive ovarian cancer model, so the PEO1/PEO1-CDDP pair provides a defined system for comparing cisplatin-sensitive and acquired cisplatin-resistant states within the same parental background.
Gender: Female
Production details: The PE01CDDP cell line was established by in vitro exposure of the PE01 line to cisplatin commencing at 25 nmol/L cisplatin and increasing to 1 Amol/L over a period of 4 months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further 4 months to ensure that the resistance remained stable. PEO1 CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines (ECACC Catalogue numbers 10032308, 10032309 & 10032310).
Cellosaurus ID: CVCL_Y032
Parental cell line: PEO1
Disease: Cancer
Related tools: 151672 ; 151673 ; 151674 ; 162283
Cat. #: 151784
Provenance and Ownership: The PEO1-CDDP Cell Line is owned by Cancer Research UK. CancerTools supplies the PEO1-CDDP Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing PEO1-CDDP Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer; Cell signaling and signal transduction; Drug development
Organism: Human
Tissue: Ovary
Gender: Female
Model: Cisplatin Resistance
Cancer Types In Detail: Adenocarcinoma
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 containing 10% heat-inactivated FCS, penicillin (100 units/mL), and streptomycin (100 ug/mL). Cells were maintained routinely at 37°C in a humidified atmosphere of 5% CO2 in air.
Temperature: 37° C
Atmosphere: 5% CO2
References:

Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.

Images

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PEO1-CDDP Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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