Cat. #161802
Osteopontin null squamous cell carcinoma cell line
Cat. #: 161802
Availability: 8-10 weeks
Organism: Mouse
Tissue: Skin
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pi-Ling Chang and Yu-Hua Hsieh
Institute: The UAB Research Foundation
Primary Citation: Hsieh et.al. 2012. Int J Cancer. PMID: 21826648
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Osteopontin null squamous cell carcinoma cell line
- Cancer: Skin cancer
- Research fields: Cancer
- Organism: Mouse
- Gender: Female
- Tissue: Skin
- Donor: Female OPN-null mouse subjected to two-stage skin carcinogenesis
- Growth properties: Adherent
- Crispr: No
- Receptors of note: No
- Description: Cells derived from a female mouse with cutaneous squamous cell carcinoma (SCC) lacking osteopontin (OPN) gene. Cells contain p53 and H-ras mutations which are common characteristics of SCCs. These cells develop SCC in syngeneic wild-type and OPN-null mice. Cell line is great for studying host-derived OPN at the systemic and tumor microenvironmnet level in the context of extrinsic cancer cell progression in immune-competent mice
- Production details: OPN-null and WT mice in 129S6/SvEv background were bred in-house. ONSC cells were derived from an SCC developed in a female OPN-null mouse subjected to two-stage skin carcinogenesis. Cells were maintained in high-glucose, low-calcium DMEM containing 10% fetal bovine serum.
Handling
- Growth medium: DMEM with 10% fetal bovine serum
References
- Hsieh et.al. 2012. Int J Cancer. PMID: 21826648
