#162155

METTL14-AID cell line

Cat. #162155

METTL14-AID cell line

Cat. #: 162155

Sub-type: Primary

Organism: Mouse

Tissue: Blastocyst

Model: Genetically modified cell line; Knock in

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Institut Curie

Primary Citation: Tomasz Chelmicki et. al. 2021 Nature. 591(7849):312-316. PMID: 33442060

Tool Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: METTL14-AID cell line
  • Research fields: Stem cell biology
  • Tool sub type: Primary
  • Parental cell: E14 murine ES cells (129Ola strain)
  • Organism: Mouse
  • Gender: Male
  • Tissue: Blastocyst
  • Growth properties: Adherent
  • Model: Genetically modified cell line; Knock in
  • Model description: The cell line contains OsTIR1 at the TIGRE locus. The AID tag is inserted in the N terminus of the Mettl14 gene.
  • Crispr: Yes
  • Conditional description: Depletion is achieved upon Auxin treatment (degradation of the AID-tagged proteins)
  • Description: Murine embryonic stem cells with an inducible and reversible (via degron AID) KO of Mettl14 - protein used for m6A methylation of RNA

Handling

  • Format: Frozen
  • Passage number: P5
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice
  • Cultured in antibiotics: Yes
  • Characterisation tests: Western blotting to confirm the depletion of the protein

Related Tools

  • Related tools: Mettl3-AID; Metll14-AID cell line and Mettl3-AID cell line

References

  • Tomasz Chelmicki et. al. 2021 Nature. 591(7849):312-316. PMID: 33442060