Melb-s3 cell line

Immortal melanoblast cell lines derived from the skin of neonatal Ednrbs/Ednrbs C3H mice

Contributors

Inventor Institute
Elena Sviderskaya St. George's University London
Cat. #: 162045
Organism: Mouse
Tissue: Skin
Model: Immortalised non-cancerous cell lines
Morphology: Small, oval-fusiform
Product description: The melb-s1, -s2 and s3 lines are immortal melanoblast cell lines derived from the skin of neonatal Ednrbs/Ednrbs C3H mice. These cells express dopachrome tautomerase (also referred to as tyrosinase-related protein 2) but generally do not express tyrosinase. These cells are unpigmented. Melanocytes, irreversibly differentiated from melb-s1 melanoblasts (melan-s1) are also available from CancerTools.org. This melanoblast line is useful for developmental studies and, as melanoblasts display mechanisms similar to tumour cells (migration to and invasion of the epidermis) they may be a useful tool in studies of melanoma development.
Storage conditions: liquid N2
Growth medium: RPMI + FBS (10%) + fibroblast growth factor 2 (FGF2) (40 pM) + mouse stem cell factor (mSCF) (20 ng/ml)

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