#162054

Melan-Gnas1 cell line

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Cat. #162054

Melan-Gnas1 cell line

Cat. #: 162054

Organism: Mouse

Tissue: Skin

Disease: Non-cancer

Model: Immortalised non-cancerous cell lines

£575.00

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Contributor

Inventor: Elena Sviderskaya

Institute: St. George's University London

Primary Citation: Unpublished

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Melan-Gnas1 cell line
  • Organism: Mouse
  • Tissue: Skin
  • Disease: Non-cancer
  • Morphology: Small, oval-fusiform
  • Model: Immortalised non-cancerous cell lines
  • Description: The melan-Gnas1, -Gnas2 and Gnas-3 cell lines are immortal melanocytes derived from Gnas-mutant Ink4aArf -/- C3H/C57BL/6J OlaHsd mice. The Gnas gene encodes the G?s protein, a subunit of the heterotrimeric G-protein complex that activates adenylate cyclase and increases cAMP production. Mutations in the human GNAS gene are found in pituitary tumours and result in constitutively activated G?s. Somatic gain-of-function mutations in GNAS are associated with McCune-Albright Syndrome, a rare disorder characterised by fibrous dysplasia of the bone, various endocrinopathies and hyperpigmented patches of skin. These murine melanocytes are hyperpigmented compared to littermate control lines. These melanocytes are a useful tool for studies of pigmentation genetics and for the effects of constitutively active cAMP signalling. Littermate control lines (melan-a9, -a10 and -a11) are also available from CancerTools.org.
  • Application: Melanocyte cell biology

Applications

  • Application: Melanocyte cell biology

Handling

  • Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
  • Storage conditions: liquid N2

References

  • Unpublished