MDA-MB-231 CAGA12-Luc/TK Renilla cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MDA-MB-231 CAGA12-Luc/TK Renilla cell line
• Cancer type filter: Breast cancer
• Cancers detailed: Breast cancer
• Research fields: Cancer;Cell signaling and signal transduction;Developmental biology
• Tool sub type: Continuous
• Parental cell: MDA-MB-231 cell line
• Organism: Human
• Tissue: Breast
• Disease: Cancer
• Model: Reporter
• Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
• Description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
• Production details: Made by transfecting the breast cancer cell line MDA-MB-231 with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Clonal
• Biosafety level: 1
• Recommended controls: MDA-MB-231 parental line

Target Details

• Target: Activin and transforming growth factor Î?‹Â› (TGF-Î?‹Â›)Â?‚ signalling

Handling

• Format: Frozen
• Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: HEK293T CAGA12-Luc/TK Renilla cell line

References

• Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.