#156439

MDA-MB-231 BRE-Luc/TK Renilla cell line

Cat. #156439

MDA-MB-231 BRE-Luc/TK Renilla cell line

Cat. #: 156439

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Reporter

£430.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Caroline Hill

Institute: The Francis Crick Institute

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MDA-MB-231 BRE-Luc/TK Renilla cell line
  • Tool sub type: Continuous
  • Parental cell: MDA-MB-231 cell line
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Reporter
  • Conditional description: Luciferase is expressed in the presence of bone morphogenetic protein (BMP) signalling
  • Description: The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for bone morphogenetic protein (BMP) signalling. The cell line stably expresses BMP responsive element (BRE)-Luciferase. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control. BMP is a transforming growth factor ? (TGF-?) superfamily member.
  • Production details: Made by transfecting the breast cancer cell line MDA-MB-31 with BRE-Luciferase plasmid, TK Renilla plasmid and pRetroSuper for puromycin resistance. Clonal
  • Biosafety level: 1
  • Recommended controls: MDA-MB-231 parental line

Target Details

  • Target: Bone morphogenetic protein (BMP) signalling

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 ?g/ml blasticidin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: HEK293T BRE-Luc/TK Renilla cell line

References

  • Grnroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.