MCF10A-PTENm-5 cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MCF10A-PTENm-5 cell line
• Alternate name: 06M17013
• Cancers detailed: Non-tumorigenic
• Research fields: Cancer;Cell biology
• Tool sub type: Continuous
• Parental cell: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
• Clone: PTEN_type-12
• Organism: Human
• Tissue: Mammarygland epithelium
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Mutant
• Model description: Type-12; Type mutation: R335*; CDS mutation: c.1003C>T; AA mutation: p.R335*; COSMIC Mutation ID: -
• Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, PTEN (phosphatase and tensin homolog). PTEN is a multi-functional tumor suppressor that is mutated and lost in a large number of cancers at high frequency. Observed in prostate cancer, glioblastoma, endometrial, lung and breast cancer to varying degrees. Up to 70% of prostate cancer patients have been observed to have loss of expression of the gene. It is a part of the PI3K/AKT/mTOR pathway and mTOR inhibitors have been relatively ineffective in treating patients with PTEN loss.
• Application: Functional analysis of mutated genes, Drug screening
• Biosafety level: 1

Target Details

• Target: Phosphatase and tensin homolog [PTEN]
• Target background: Gene ID: 5728; References: DNA (mRNA): NM_000314.6; Protein: NP_000305.3

Applications

• Application: Functional analysis of mutated genes, Drug screening

Handling

• Format: Frozen
• Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
• Temperature: 37° C
• Atmosphere: Humidified incubator with 5% CO2
• Shipping conditions: Dry ice
• Storage medium: CELLBANKER 2 (Zenogen pharma)
• Storage conditions: Liquid Nitrogen
• Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
• Cultured in antibiotics: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
• Mycoplasma free: Yes
• Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests