#161208

MCF10A-PTENm-5 cell line

Cat. #161208

MCF10A-PTENm-5 cell line

Cat. #: 161208

Sub-type: Continuous

Organism: Human

Tissue: Mammarygland epithelium

Model: Mutant

£575.00

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Contributor

Inventor: Medical-Industrial Translational Research Center

Institute: Fukushima Medical University

Tool Details
Target Details
Applications
Handling

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MCF10A-PTENm-5 cell line
  • Alternate name: 06M17013
  • Cancers detailed: Non-tumorigenic
  • Research fields: Cancer;Cell biology
  • Tool sub type: Continuous
  • Parental cell: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
  • Clone: PTEN_type-12
  • Organism: Human
  • Tissue: Mammarygland epithelium
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Mutant
  • Model description: Type-12; Type mutation: R335*; CDS mutation: c.1003C>T; AA mutation: p.R335*; COSMIC Mutation ID: -
  • Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, PTEN (phosphatase and tensin homolog). PTEN is a multi-functional tumor suppressor that is mutated and lost in a large number of cancers at high frequency. Observed in prostate cancer, glioblastoma, endometrial, lung and breast cancer to varying degrees. Up to 70% of prostate cancer patients have been observed to have loss of expression of the gene. It is a part of the PI3K/AKT/mTOR pathway and mTOR inhibitors have been relatively ineffective in treating patients with PTEN loss.
  • Application: Functional analysis of mutated genes, Drug screening
  • Biosafety level: 1

Target Details

  • Target: Phosphatase and tensin homolog [PTEN]
  • Target background: Gene ID: 5728; References: DNA (mRNA): NM_000314.6; Protein: NP_000305.3

Applications

  • Application: Functional analysis of mutated genes, Drug screening

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
  • Temperature: 37° C
  • Atmosphere: Humidified incubator with 5% CO2
  • Shipping conditions: Dry ice
  • Storage medium: CELLBANKER 2 (Zenogen pharma)
  • Storage conditions: Liquid Nitrogen
  • Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
  • Cultured in antibiotics: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
  • Mycoplasma free: Yes
  • Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests