Immorta-MAIT T cell clone MG-A11 cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Immorta-MAIT T cell clone MG-A11 cell line
• Research fields: Immunology
• Organism: Human
• Disease: Mycobacterium tuberculosis
• Growth properties: TRAV1-2 is expressed uniformly (determined by flow cytometry staining). This clone expresses CD4, CD8, CD161, and CD27 and can be classified as a double-positive thymocyte. This T cell clone binds the MR1/5-OP-RU tetramer but not MR1/6FP tetramer (negative control). Clone is MR1-restricted in its production of IFN-gamma by ELISPOT test, determined by its response to M. smegmatis-infected A549 cell line but not a M. smegmatis-infected MR1-- A549 cell line.
• Model: Immortalised Line
• Conditional: No
• Description: Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in autoimmune diseases such as multiple sclerosis, rheumatiod arthritis, and systemic lupis erythematosus.
• Production details: Generated by single cell isolation of MR1/5-OP-RU tetramer+ cells from a human thymus. Single cells were rapidly expanded into a T cell clone using antibody to CD3 (clone OKT3) and IL-2.

Handling

• Format: Frozen
• Growth medium: These cell lines are not suitable for large-scale expansion following standard cell culture protocols. Following the recommended guidelines the cells should double after a week, after which cells are stable and useable for weeks.
  
  Upon receipt spin cells and resuspend them in 15ml of fresh media and add to T12.5 flask. Recommended media is RPMI supplemented with 10% FBS, L-Glut, gentamicin and 0.5ng/mL IL2. Incubate at 37 °C. Aspirate half the media and replace with fresh media every 3-4 weeks.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice