IDHmut glioma stem-like NCH3763 cell line

Glioma stem-like cell line NCH3763 expressing mutant isocitrate dehydrogenase (IDH), used to model low-grade gliomas or secondary glioblastomas

Contributors

Inventor Institute
Christel Herold-Mende Heidelberg University
Cat. #: 162307
Cancer types: Brain
Organism: Human
Tissue: Brain
Morphology: Grown as free-floating neurospheres under serum-free culture conditions.
Growth properties: Cells grow as free-floating neurospheres under serum-free conditions in non-adherent culture vessels. Cultures are maintained at 37°C in a humidified incubator with 5% CO2.
Primary citation: Trong et al. 2023. Cells. 12(8):1200. PMID: 37190109.
Product description: Glioma stem-like cell line NCH3763 expressing mutant isocitrate dehydrogenase (IDH), used to model low-grade gliomas or secondary glioblastomas
Production details: Patient-derived cell line established from human tumour tissue obtained during surgical resection and expanded in vitro.
Storage conditions: FCS-free freezing medium (Synth-a-freeze medium)
Growth medium: DMEM/F-12–based serum-free medium supplemented with BIT, GlutaMAX, EGF, and FGF-2.
Subculture routine: Neurospheres are passaged at high cell density by gentle mechanical dissociation. Accutase may be used if required to obtain single-cell suspensions. Cells are reseeded in fresh serum-free medium.
Temperature: 37°C
References:

Trong et al. 2023. Cells.12(8):1200. PMID: 37190109.

Trong et al. 2020. Cells. 9(6):1389. PMID: 32503220.

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