Human Melanocyte [bFGF] Cell Line

Melanocytes are the cell of origin for a number of conditions including melanoma an aggressive type of skin cancer and vitiligo a long-term condition characterised by patches of the skin losing their pigment. Melanoma is the most dangerous type of skin cancer. Globally, in 2012, it newly occurred in 232,000 people. In 2015 there were […]

Contributors

Inventor
Pranab K Das
Cat. #: 154111
Tool sub type: Primary
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell biology
Organism: Human
Tissue: Skin
Model: Primary line
Product description: Melanocytes are the cell of origin for a number of conditions including melanoma an aggressive type of skin cancer and vitiligo a long-term condition characterised by patches of the skin losing their pigment. Melanoma is the most dangerous type of skin cancer. Globally, in 2012, it newly occurred in 232,000 people. In 2015 there were 3.1 million with active disease which resulted in 59,800 deaths. Australia and New Zealand have the highest rates of melanoma in the world. While Vitiligo affects 1% of the worlds population with no known cause. Primary melanocytes can be used as a tool for research into different melanocytic lineages
Conditional: No
Production details: Melanocyte cultures were established from neonatal foreskin using standard methods. Culture medium was supplemented with bFGF.
Parental cell line: Human foreskin
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 100ng/ml bFGF, 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100g/ml streptomycin
Mycoplasma free: Yes
Biosafety level: 1

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