HT-29 Cell Line

HT-29 is a widely used human colorectal adenocarcinoma cell line, exhibiting sensitivity to chemotherapeutic agents such as 5-fluorouracil (5FU) and oxaliplatin. These cells display phenotypic characteristics of mature intestinal cells, including enterocytes, making them a well-established in vitro model for studying food digestion and bioavailability. Additionally, HT-29 cells are used in drug discovery and microbiological […]

Contributors

Inventor Institute
Jorgen Fogh Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161929
Cancer types: Colorectal cancer
Tissue: Colon tissue
Gender: Female
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59.1.221. PMID: 327080.
Alternate name: HT29; HT 29
Product description: HT-29 is a widely used human colorectal adenocarcinoma cell line, exhibiting sensitivity to chemotherapeutic agents such as 5-fluorouracil (5FU) and oxaliplatin. These cells display phenotypic characteristics of mature intestinal cells, including enterocytes, making them a well-established in vitro model for studying food digestion and bioavailability. Additionally, HT-29 cells are used in drug discovery and microbiological research to investigate probiotics and bacteriophage therapies.
Gender: Female
Disease: Adenocarcinoma;Colorectal Cancer
Products or characteristics of interest: Karyotype: hypertriploid, modal number = 71; range = 68 to 72. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 2; PGM1, 1-2; PGM3, 2
Shipping conditions: Dry Ice
Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% DMSO
Biosafety level: 1
References:

Nautiyal et al. 2011. Journal of molecular signaling. 6:7. PMID: 21774804.

Cohen et al. 1999. Journal of cell science. 112.16.2657. PMID: 10413674.

Images

View Gallery
HT-29 colorectal adenocarcinoma cell line (Cat. #: 161929) shown at low magnification. Image courtesy of Memorial Sloan Kettering Cancer Center.
HT-29 colorectal adenocarcinoma cell line (Cat. #: 161929) shown at high magnification. Image courtesy of Memorial Sloan Kettering Cancer Center.

Citations And Reviews

Related Tools for Cell lines

Cat. # Tool Name
151446 FLYRD18 Cell Line Key Info View Tool
151579 U-2 OS Gal4-p300 Cell Line Key Info View Tool
151630 PDVC57B Cell Line Key Info View Tool
151631 C-Raf KI D486A Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.