Cat. #153209
HCT 116 ADAM17 Cell Line
Cat. #: 153209
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Colon
Disease: Cancer
Model: Knock-In
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sandra Van Schaeybroeck
Institute: Queen's University Belfast
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HCT 116 ADAM17 Cell Line
- Cancer: Digestive/Gastrointestinal cancer
- Cancers detailed: Colorectal
- Research fields: Cancer;Drug development
- Tool sub type: Continuous
- Parental cell: HCT 116
- Organism: Human
- Tissue: Colon
- Disease: Cancer
- Growth properties: Invasion, migration
- Model: Knock-In
- Conditional: No
- Description: Chemotherapy (5-fluorouracil) treatment has been shown to result in acute increases in transforming growth factor-a, amphiregulin, and heregulin ligand shedding in vitro and in vivo correlating with significantly increased ADAM-17 activity. Small interfering RNAmediated silencing and pharmacologic inhibition confirmed that ADAM-17 was the principal ADAM involved in this prosurvival response. HCT 116 ADAM17 Cell Line showed that overexpression of ADAM-17 significantly decreases the effect of chemotherapy on tumor growth and apoptosis.
- Production details: HCT116 cells were cotransfected with 10 ÄÂÄ?g of a plasmid containing hemagglutinin (HA)-tagged full-length mouse ADAM-17 (HA-ADAM-17) and a construct expressing a puromycin resistance gene. Stably transfected cells were selected and maintained in medium supplemented with 1 ÄÂÄ?g/mL puromycin.
- Additional notes: Offered under licence from the Spanish National Research Council (CSIC)
- Recommended controls: HCT 116 parental line
- Cellosaurus id: CVCL_HG03
Target Details
- Target: mouse ADAM17 (TACE)
Handling
- Format: Frozen
- Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Kyula et al. 2010. Clin Cancer Res. 16(13):3378-89. PMID: 20570921.
- Chemotherapy-induced activation of ADAM-17: a novel mechanism of drug resistance in colorectal cancer.
