#151681

Gamma2A Jak2 Cell Line

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Cat. #151681

Gamma2A Jak2 Cell Line

Cat. #: 151681

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Disease: Cancer

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: George Stark ; Ian Kerr

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Gamma2A Jak2 Cell Line
  • Cancer: Sarcoma
  • Cancers detailed: Fibrosarcoma
  • Research fields: Cell signaling and signal transduction;Immunology
  • Tool sub type: Continuous
  • Parental cell: HT 1080
  • Organism: Human
  • Disease: Cancer
  • Model: Transgenic
  • Description: The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase dom...
  • Production details: The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2.
  • Biosafety level: 1
  • Recommended controls: HT 1080 parental line
  • Cellosaurus id: CVCL_1D51

Target Details

  • Target: Gamma2A Jak2

Handling

  • Format: Frozen
  • Growth medium: DMEM with 10% v/v heat-inactivated FCS and 400ug/ml G418 plus 0.5 ug/ml puromycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: 2C4-Gamma2A cell line ; 2C4 cell line

References

  • Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.
  • JAK/STAT-deficient cell lines.
  • Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
  • A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
  • Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
  • Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.