Gamma2A Jak2 Cell Line

The Gamma2A Jak2 Cell Line lacks inducible phosphorylation of the IFN-gamma receptor 1 (R1) subunit, JAK1, and STAT1 and IFN-gamma-inducible cell surface.

Contributors

Inventor Institute
George Stark ; Ian Kerr Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151681
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Sarcoma
Research Fields: Cell signaling and signal transduction;Immunology
Organism: Human
Model: Transgenic
Cancer Types In Detail: Fibrosarcoma
Product description: The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase dom…
Production details: The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2.
Cellosaurus ID: CVCL_1D51
Parental cell line: HT 1080
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM with 10% v/v heat-inactivated FCS and 400ug/ml G418 plus 0.5 ug/ml puromycin
Recommended controls: HT 1080 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.

JAK/STAT-deficient cell lines.

Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.

A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.

Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.

Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.

Related Tools for Cell lines

Cat. # Tool Name
151446 FLYRD18 Cell Line Key Info View Tool
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151630 PDVC57B Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.