Serum Free-Suspension adapted recombinant CHO-K1 cell line with stable production of second generation Human Erythropoietin protein called Darbepoetin (Darbe)

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Serum Free-Suspension adapted recombinant CHO-K1 cell line with stable production of second generation Human Erythropoietin protein called Darbepoetin (Darbe)
• Alternate name: haematopoietin, or haemopoietin
• Research fields: Drug development
• Parental cell: CHO-K1
• Organism: Hamster
• Tissue: Ovary
• Donor: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbepoetin gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The Darbepoetin is expressed without any affinity tag and is actively secreted in the culture supernatant. The adherent cells were adapted in serum free media (CD Forti media) and grown in shake flask.
• Disease: Therapeutics
• Conditional: No
• Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Darbepoetin gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The Darbepoetin is expressed without any affinity tag and is actively secreted in the culture supernatant. The adherent cells were adapted in serum free media (CD Forti media) and grown in shake flask.

Target Details

• Target: Erythropoietin (EPO), Darbepoetin (Darbe)

Handling

• Growth medium: Grown in Serum Free CD Forti media. The cells are grown in a CO2 incubator at 37?‚°C with 5% CO2 with shaking.
• Temperature: 37° C
• Atmosphere: 5% CO2

References

• A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)