Capan-1 Cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Capan-1 Cell line
• Organism: Human
• Gender: Male
• Tissue: Pancreas
• Donor: 40-year-old, White, male with pancreatic adenocarcinoma
• Morphology: Epithelial
• Growth properties: Adherent
• Products or characteristics of interest: Expression Markers: Progesterone receptor, positive; estrogen receptor, alpha, positive. Karyotype: (P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker. Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1-2; PGM3, 1
• Description: Capan-1 is a human pancreatic ductal adenocarcinoma cell line. These cells grow in adherent tissue culture and display epithelial morphology. In culture, these cells are capable of invading through an extracellular matrix, such as Matrigel. The Capan-1 cells are resistant to 5-fluorouracil, reminiscent of the original tumour from which they were derived. They form poorly-differentiated tumours when injected into immunocompromised mice. These cells harbour a single base-pair deletion in the BRCA2 allele, which results in the expression of a truncated and dysfunctional protein. In addition, they have an oncogenic mutation in K-Ras (G12V) and an inactivating mutation in p53. These cells express elevated levels of the Epidermal Growth Factor Receptor (EGFR) and do not express SMAD4 protein (i.e., SMAD4-null). The Capan-1 cells are useful both as a xenograft model for pancreatic cancer and as a cell system to study the effects of BRCA2-deficiency.
• Application: 3D cell culture; Cancer research
• Biosafety level: 1

Target Details

• Target: Antigen Expression: Blood Type A; Rh+; HLA A2, A9, B13, B17
• Target background: Genes expressed: mucin; blood type A; Rh+; HLA: (A2; A9; B13; B17.

Applications

• Application: 3D cell culture; Cancer research
• Application notes: The cells will slough off of the growth surface if they become too heavy. Capan-1 expresses the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete gastric type mucins.

Handling

• Growth medium: IMDM supplemented with 20% FBS
• Temperature: 37° C
• Shipping conditions: Dry Ice
• Storage medium: Culture medium 95%; DMSO, 5%
• Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
• Str profiling: D3S1358: 15;TH01: 6;D21S11: 28,30;D18S51: 12;Penta_E: 10,12;D5S818: 11;D13S317: 9;D7S820: 10,11;D16S539: 13,14;CSF1PO: 11;Penta_D: 9,13;Amelogenin: X;vWA: 16;D8S1179: 14,16;TPOX: 8,11;FGA: 24;D19S433: 14,15;D2S1338: 20,24

References

• Fogh et al.1977.Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
• Kyriazis et al. 1982. American Journal of Pathology 106: 250-260. PMID: 6278935.
• Deer et al. 2010. Pancreas. 39: 425-435. PMID: 20418756.