Calu-3 Cell line

Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene. They express wildtype EGFR and mutant K-Ras (G13D). In addition, they harbour mutations in TP53 and CDKN2A genes. The Calu-3 cells are sensitive to erlotinib […]

Contributors

Inventor Institute
Jorgen Fogh, Germain Trempe Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161906
Cancer types: Lung cancer
Organism: Human
Tissue: Lung
Gender: Male
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Fogh J et al.1977.Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.
Product description: Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene. They express wildtype EGFR and mutant K-Ras (G13D). In addition, they harbour mutations in TP53 and CDKN2A genes. The Calu-3 cells are sensitive to erlotinib (EGFR tyrosine kinase inhibitor) and cetuximab (a monoclonal antibody that blocks ligand binding to EGFR and prevents downstream signalling), two commonly used drugs targeting ErbB receptors. These cells are capable of forming tumours in immunocompromised mice.
Gender: Male
Products or characteristics of interest: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1; PGM1, 1; PGM3, 1-2; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 1-2; PGM1, 1-2; PGM3, 1
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: EMEM suplemented with 10% FBS.
Subculture routine: 1. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 2. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Temperature: 37° C
Atmosphere: 95% Air, 5% CO2
Storage medium: Culture medium 95%; DMSO, 5%
Biosafety level: 1
References:

Fogh J et al.1977.Journal of the National Cancer Institute. 59: 221-226.PMID: 327080.

Cavazzoni A et al. 2007. Cancer Letters. 246: 69-81. PMID: 16616810.

Blanco R et al. 2009. Human Mutation. 30: 1199–1206. PMID: 19472407.

Related Tools for Cell lines

Cat. # Tool Name
151445 1-7HB2 Cell Line Key Info View Tool
151447 CMT 64 Cell Line Key Info View Tool
151665 MEF PKCe KO Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool
151655 FLYA-13 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.