C75 Colorectal adenocarcinoma Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: C75 Colorectal adenocarcinoma Cell Line
• Alternate name: C75 Cell Line
• Cancer: Adenocarcinoma
• Cancers detailed: Adenocarcnoma of the sigmoid colon
• Research fields: Cancer;Drug development
• Organism: Human
• Tissue: Colon
• Disease: Cancer
• Model: Tumour line
• Conditional: Yes
• Description: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes' stage D.
• Cellosaurus id: CVCL_5248

Handling

• Format: Frozen
• Growth medium: Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
• Temperature: 37° C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Subculture routine: Cells grow as small dense islands and will not form a monolayer. C75 cells grow very slowly. Recommended that the cell line should be subcultured approximately every 5 days. On resuscitation, Centrifugation of the cells (100g x 5 min) to remove DMSO improves the establishment of a viable culture. Seed cells at 2-4x10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA as a dissociation reagent. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells. Following resuscitation or subculture, the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Recommended growth conditions 5% CO ; 37°C.
• Mycoplasma free: Yes

References

• Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.