A549 LIMD1 KO Cas9 cell line

A549 lung adenocarcinoma line knocked-out for the tumour suppressor gene LIMD

Contributors

Inventor Institute
Tyson Sharp Queen Mary University of London
Cat. #: 162160
Research Fields: Cancer
Organism: Human
Tissue: Lung
Gender: Male
Model: Genetically modified cell line; Knock out
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Lung Cancer
Primary citation: Kathryn Davidson et. al. 2021. Cell Death Dis. 12(11): 1075. PMID: 34764236
Product description: A549 lung adenocarcinoma line knocked-out for the tumour suppressor gene LIMD1, which is commonly lost as an early event in lung oncogenesis. This gene encodes a scaffold protein suppressesing tumorigenesis via a number of different mechanisms. This line has been used to test drugs in LIMD1-deficient lung cancers, both in vitro an in vivo. When inoculated into NOD/SCID mice subcutaneously, CRISPR-Cas9-generated LUAD A549 LIMD1+/+ and LIMD1?/? cells generate LIMD1+/+ and LIMD1?/? tumours, respectively.
Gender: Male
CRISPR: Yes
Parental cell line: A549 cell line
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM medium supplemented with 10% FCS and 1% penicillin/streptomycin solution
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

Kathryn Davidson et. al. 2021. Cell Death Dis. 12(11): 1075. PMID: 34764236

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