1BR3 PBRM1 KO5 + PBRM1

1BR3-hTERT cell line knockout for PBRM1 (clone 5) stably expressing DOX-inducible PBRM1 construct

Contributors

Inventor Institute
Jessica Downs, Hugang Feng The Institute of Cancer Research
Cat. #: 161870
Organism: Human
Tissue: Skin
Gender: Male
Model: Genetically modified; immortalised non-cancer cells
Morphology: Fibroblast
Growth properties: Adherent
Primary citation: Feng et. al. 2022 Genes Dev 36:790-806 PMID: 35902118
Product description: 1BR3-hTERT cell line knockout for PBRM1 (clone 5) stably expressing DOX-inducible PBRM1 construct. This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
Gender: Male
CRISPR: Yes
Conditional: Yes
Conditional description: Conditional expression of wild type PBRM1 gene following deoxycycline treatment
Parental cell line: 1BR3-hTERT
Model description: Conditional re-expression of wild type PBRM1 gene
Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM + 10S + 1% Penicillin/Streptomycin
Temperature: 37C
Atmosphere: 5% CO2 in air
Biosafety level: 1
References:

Feng et. al. 2022 Genes Dev 36:790-806 PMID: 35902118

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